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A Combined Protocol for PCR Detection of GM in Seed - VS0136
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Description
This work involves the development, optimisation and assessment of a combined PCR based approach to seed testing which can provide both % GM seed and % GM DNA results in order to improve the reliability of borderline test decisions and provide results to inform decisions for seed and crop thresholds, which may be expressed differently in legislation. The required development and testing of the protocol will also provide accurate estimates of uncertainty associated with different thresholds and measured % GMO results. |
Objective
1. Obtain GM materials for use as standard genotypes in protocol testing with known variable copy number and genotype. Establish reliable PCR tests for these lines.
2. Make mixtures of seed with variable % GM seed and known % GM DNA. Theoretically examine the statistics behind optimisation of seed bulk size and number with respect to accuracy and cost.
3. Test seed mixtures for expected binomial distribution of positive tests in stage 1 of protocol. Statistically test results and establish best size and number of Stage 1 bulks.
4. Optimise stage 2 real-time real-time PCR using seed bulks of known GM composition.
5. Combined method testing. Using materials and optimised PCRs from objectives 1-4, replicate the entire procedure several times to measure accuracy of accept/reject decisions. Compare results statistically to expected values. |
Related Documents
• ROAME Document : TESTING PLANT VARIETIES AND SEEDS |
Time-Scale and Cost
From:
2005
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To:
2006
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Cost: £49,346 |
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Contractor / Funded Organisations
Central Science Laboratory |
Keywords
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Fields of Study and Contacts
Plant Varieties and Seeds - |